Regeneration of tea [<em>Camellia sinensis</em> (L.) Kuntze] cv. TRI 5001 plants through somatic embryogenesis in a liquid dynamic medium
نویسندگان
چکیده
Somatic embryogenesis is an efficient micro-propagation technique, which can lead to mass multiplication of tea with a tap root. Hence, the development viable somatic and protocol through liquid culture conditions needed increase efficiency protocol. Cotyledon-derived embryos, leaf stem callus TRI 5001 cultivar, were tested different MS media. A significantly higher cotyledon-derived embryo rate was observed in +3 mg/L Thidiazuron (TDZ) medium. Callus proliferation compact embryonic formation comparatively + 1.1 TDZ, 1.86 NAA medium, subsequent 0.1 NAA,1 BAP 0.2 GA3 medium on produced explants. Higher nodal recorded MS+0.11 TDZ IBA MS+0.1 Plant regeneration resulted MS+3 mg/LBAP Identified treatment combinations be used as foundation establish explants tea.
منابع مشابه
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ژورنال
عنوان ژورنال: Tropical agricultural research
سال: 2022
ISSN: ['1016-1422']
DOI: https://doi.org/10.4038/tar.v33i4.8520